the in vitro effect of leptin on semen quality of water buffalo (bubalus bubalis) bulls
نویسندگان
چکیده
the purpose of this study was to evaluate the probable effects of leptin addition in different levels to the semen extender on sperm quality (motility and motility parameters, viability, sperm membrane integrity, and dna damage). semen specimens were evaluated immediately after leptin addition, equilibration time and after thawing the frozen semen. five healthy buffalo bulls (5 ejaculates from each bull) were used. each ejaculate was diluted at 37 ˚c with tris-based extender containing 0 (control), 10, 20, 50, 100, and 200 ng ml-1 leptin. the diluted semen was kept 4 hr in refrigerator to reach to the equilibration time and then packed in 0.5 ml french straws and frozen in liquid nitrogen. our results showed that, in the fresh semen, no significant difference was observed in all sperm quality parameters evaluated among all of the examined leptin concentrations. addition of 10 ng ml-1 leptin into semen extender significantly preserved sperm motility, all of the motility parameters, and viability in equilibrated semen compared to that of control group. however, in vitro addition of 200 ng ml-1 leptin, significantly decreased theses parameters. in the frozen thawed semen, all leptin concentrations decreased sperm motility and viability, but significant decrease was observed in concentrations of 100 and 200 ng ml-1. adding leptin to semen extender did not have any significant influence on sperm dna damage and sperm membrane integrity in all examined groups. these findings suggest that in vitro addition of 10 ng ml-1 leptin could preserve sperm motility and viability in cooled semen of buffaloes.
منابع مشابه
The in vitro effect of leptin on semen quality of water buffalo (Bubalus bubalis) bulls
The purpose of this study was to evaluate the probable effects of leptin addition in different levels to the semen extender on sperm quality (motility and motility parameters, viability, sperm membrane integrity, and DNA damage). Semen specimens were evaluated immediately after leptin addition, equilibration time and after thawing the frozen semen. Five healthy buffalo bulls (5 ejaculates from ...
متن کاملThe in vitro effect of leptin on semen quality of water buffalo (Bubalus bubalis) bulls
The purpose of this study was to evaluate the probable effects of leptin addition in different levels to the semen extender on sperm quality (motility and motility parameters, viability, sperm membrane integrity, and DNA damage). Semen specimens were evaluated immediately after leptin addition, equilibration time and after thawing the frozen semen. Five healthy buffalo bulls (5 ejaculates from ...
متن کاملP-117: The In Vitro Effect of Leptin on Semen Quality of Water Buffalo (Bubal Us Bubalis) Bulls
Background: Several studies have recently investigated the role of leptin, the polypeptide hormone secreted primarily by white adipose tissue, in the reproduction of rodents, humans, and domestic animals. Materials and Methods: To investigate the in vitro effect of leptin on buffalo semen quality in equilibrated and frozen thawed semen, five healthy buffalo bulls (5 ejaculates from each bull) w...
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In order to determine iron and lead content of seminal plasma in water buffalo and to study their associations with the semen characteristics, 54 semen samples were collected from 10 buffalo bulls. The semen characteristics were evaluated; its iron and lead content were estimated by atomic absorption spectrophotometer. The iron and lead content of the seminal plasma (Mean ± SEM) was recorde...
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Effect of age and season on some semen parameters of Nili-Ravi buffalo (Bubalus bubalis) bulls
This study was carried out on 16 buffalo bulls (Bubalus bubalis) divided into four age groups, including three healthy (less than 5, 6-10, and more than 11 year old animals), and one abnormal group (6-10 year old bulls having poor semen quality). The study was undertaken for a one year period divided into five seasons (dry summer, humid summer, autumn, winter and spring). Overall semen volume w...
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عنوان ژورنال:
veterinary research forumناشر: faculty of veterinary medicine, urmia university
ISSN 2008-8140
دوره 4
شماره 1 2013
کلمات کلیدی
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